TOOLSilent LentiPrecipitating Reagent
TOOLSilent LentiPrecipitating Reagent is a mixture of polymers optimized for the precipitation of lentiviral particles. It provides a simple, fast and highly efficient method for concentrating lentiviral particles. In this simple protocol, you just mix your lentiviral supernatant with the TOOLSilent LentiPrecipitating Reagent n, incubate for a short period, and spin the mixture in a standard centrifuge. You'll increase your lentivirus titer by up to 100 fold as quick as in 4 hrs, and obtain excellent recoveries without ultracentrifugation. The TOOLSilent LentiPrecipitating Reagent is a 5x solution.
Features
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Easy-to-use
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No ultracentrifugation required.
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Concentration: up to 100 fold increase
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Easy scale up for large volumes.
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Cost effective
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Non-toxic: safe for all cell lines, including ES cells.